O-39: Evaluation the Expression of Bax,Bcl-2, p53 & Survivin After Treatment ofCryptorchidism in Mouse

Background: Experimental cryptorchidism is a common model for examining the expression and function of heat-induced spermatogenesis related genes in testis. In this study the expression of genes & proteins thatinvolved in programmed cell death after treatment of cryptorchidism was examined. Materials and Methods: To induce bilateral cryptorchid model immature mice were anesthetized and a small incision was made in the abdominal skin and peritoneum then fat pad at the upper end of testis sutured to peritoneum. After performance of two treatment methods, transplantation of germ stem cells and orchidopexy molecular examination and protein expression of Bax, p53, Bcl-2 and Survivin genes were evaluated at 2 & 8 weeks after treatment comparing with control and bilateral groups. Results: RT-PCR data showed the decreased of p53 & Bax expression as well as decreased of Bcl-2 mRNA in treatment groups especially after transplantation compared with control group. The expression of survivin 140 significantly increased after treatment, where as expression of survivin 40 was lower especially in orchidopexy group. Immunohistochemistry staining showed that the intensity of Bax expression mainly decreased in treated cryptorchid testis and rates of Bcl-2 increased significantly but expression of p53 and survivin proteins not many changed after treatment. Conclusion: These observations suggest that multiple molecular pathways participate in the germ cell apoptosis induced by treatment of cryptorchidism.